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Updated: Feb 18, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Sanchari Bhattacharyya1, Shimon Bershtein2, Eugene I Shakhnovich1
1Department of Chemistry and Chemical Biology, Harvard University, Cambridge MA, USA.
Abstract:
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.
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