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The Object Context-place-location Paradigm for Testing Spatial Memory in Mice.

Edith Lesburguères1, Panayiotis Tsokas2,3, Todd Charlton Sacktor2,4

  • 1Center for Neural Science, New York University, New York, USA.

Bio-Protocol
|November 25, 2017
PubMed
Summary
This summary is machine-generated.

This study developed a novel protocol to assess long-term spatial memory in mice, evaluating how memory for object-context and object-place associations is maintained.

Keywords:
ContextDiscrimination memoryNovelty preferenceRecognition memorySpace

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Area of Science:

  • Neuroscience
  • Behavioral Neuroscience
  • Cognitive Neuroscience

Background:

  • Long-term spatial memory is crucial for navigation and survival.
  • Protein kinase C zeta (PKMζ) is implicated in memory consolidation.
  • Understanding spatial memory maintenance requires precise behavioral assays.

Purpose of the Study:

  • To establish a comprehensive protocol for assessing long-term spatial memory maintenance in mice.
  • To investigate the sensitivity of spatial memory to the type and complexity of learned information.
  • To evaluate the role of PKMζ in maintaining object-context and object-place associations.

Main Methods:

  • A modified protocol integrating object-in-context, object-in-place, and object-in-location novelty-preference tests.
  • Mice were trained in three distinct environments with unique object arrangements.
  • Memory recall was assessed using environment mismatch tests, measuring novelty discrimination based on exploration time.

Main Results:

  • The protocol successfully differentiated between memory for object-context and object-place associations.
  • Novelty discrimination indices reflected the recall of learned spatial configurations.
  • The behavioral measure effectively captured the maintenance of spatial information.

Conclusions:

  • The developed protocol provides a robust method for studying spatial memory maintenance.
  • This assay allows for the examination of how different types of spatial information are retained over time.
  • Future studies can utilize this protocol to explore the molecular mechanisms, such as PKMζ's role, in spatial memory persistence.