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Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
The most frequent Mycobacterium tuberculosis complex families in mali (2006-2016) based on spoligotyping
Antieme Combo Georges Togo1, Ousmane Kodio1, Bassirou Diarra1
1University Clinical Research Center-SEREFO-Laboratory, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali.
Background:
To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years.
Methods:
From 2006 to 2016, we conducted a cross-sectional study to identify with spoligotyping, clinical isolates from tuberculosis (TB)-infected patients at different stages of their treatments in Bamako, Mali.
Results:
Among the 904 suspected TB patients included in the study and thereafter tested in our BSL-3 laboratory, 492 (54.4%) had MTBc and therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1 (31.9%) and MTB LAM10 (15.3%) from lineage 4 and M. africanum 2 (16.8%) from lineage 6 were the leading causes of TB in Bamako region during the past 10 years. Other spoligotypes such as MTB T3, MTB Haarlem 2, MTB EAI3, and MTB family 33 were also commonly seen from 2010 to 2016.
Conclusion:
This study showed a high genetic diversity of strains isolated in Bamako region and highlights that M. tuberculosis T1 strain was the most prevalent. Furthermore, the data indicate an increasing proportion of primary drug resistance overtime in Bamako.
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