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Related Experiment Videos

Glutamine transport by rat basolateral membrane vesicles.

F K Ghishan1, W Sutter, H Said

  • 1Department of Pediatrics and Surgery, Vanderbilt University Hospital, Nashville, TN 37232.

Biochimica Et Biophysica Acta
|February 13, 1989
PubMed
Summary

This study reveals two distinct pathways for glutamine transport across rat basolateral membranes: one sodium-dependent and electrogenic, the other sodium-independent and electroneutral. Both are carrier-mediated, saturable, and inhibited by other neutral amino acids.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Glutamine is a crucial amino acid for nitrogen transport and ammonia metabolism.
  • The gastrointestinal tract utilizes glutamine as a primary respiratory substrate.
  • Intestinal enterocytes absorb glutamine from both luminal and arterial sources via basolateral membranes.

Purpose of the Study:

  • To characterize the transport mechanisms of glutamine across the basolateral membrane of rat enterocytes.
  • To determine the kinetic properties and driving forces of glutamine uptake.
  • To investigate the electrogenic or electroneutral nature of glutamine transport pathways.

Main Methods:

  • Preparation of rat basolateral membrane vesicles using Percoll density gradient centrifugation.

Related Experiment Videos

  • Assessing glutamine uptake under varying sodium and potassium gradients.
  • Kinetic analysis of glutamine transport at different substrate concentrations.
  • Investigating the effects of membrane potential and pH on glutamine uptake.

    • Main Results:

    • Glutamine uptake is mediated by both sodium-dependent and sodium-independent carrier systems.
    • Both transport systems exhibit saturation kinetics with distinct Vmax and Km values.
    • The sodium-dependent pathway is electrogenic, while the sodium-independent pathway is electroneutral.
    • Glutamine uptake shows a pH optimum of 7.5 and is inhibited by other neutral amino acids.

    Conclusions:

    • Rat basolateral membranes employ distinct carrier-mediated processes for glutamine uptake.
    • The identified sodium-dependent and independent pathways differ in their electrogenic properties.
    • These findings elucidate the complex mechanisms governing intestinal glutamine absorption.