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Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

775.4K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Related Experiment Video

Updated: Feb 18, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

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Sanger sequencing.

Diego Estrada-Rivadeneyra1

  • 1University of Manchester, Manchester, UK.

The FEBS Journal
|November 25, 2017
PubMed
Summary

Sanger sequencing is a vital method for DNA analysis. This award-winning poster clearly explains its process and significance in molecular biology.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • The poster details Sanger sequencing, a foundational method in DNA sequencing.
  • It highlights the technique's historical and ongoing importance in genetic research.

Discussion:

  • The communication effectively visualizes the Sanger sequencing workflow.
  • It emphasizes the method's critical role in understanding genetic information.

Key Insights:

  • Sanger sequencing remains a key tool for specific DNA analysis tasks.
  • The poster serves as an excellent educational resource for this technique.

Outlook:

  • Further advancements in sequencing technologies build upon the principles of Sanger sequencing.

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  • Effective science communication is crucial for disseminating knowledge of complex biological methods.