Related Experiment Video

Updated: Feb 18, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

9.0K

Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic

Stéphanie Durrieu-Gaillard1,2, Hélène Dumay-Odelot1,2, Galina Boldina1,2,3

  • 1a Université de Bordeaux , ARNA Laboratory , F-33076 Bordeaux , France.

Cell Cycle (Georgetown, Tex.)
|November 25, 2017
PubMed

Insights

Tumorigenesis involves increased RNA polymerase III (Pol III) transcription. Upregulation of Pol III components and specific RNAs supports tumor formation, offering new insights into cancer development.

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Gene Regulation

Background:

  • RNA polymerase (Pol) III transcribes essential small untranslated RNAs.
  • Pol III activity is modulated by tumor suppressor inactivation and oncogene overexpression.
  • The combined effects of these factors on Pol III transcription during tumorigenesis are not fully understood.

Purpose of the Study:

  • To comprehensively analyze the regulation of Pol III transcription during tumorigenesis.
  • To investigate the role of Pol III system components in cellular transformation.
  • To assess if Pol III upregulation is sufficient to sustain tumor formation.

Main Methods:

  • Utilized a model system with five genetic elements for cellular transformation.
  • Analyzed six distinct transformation intermediate cell lines derived from IMR90 fibroblasts.
  • Measured RNA and protein levels of Pol III subunits and transcription factors.

Main Results:

  • Cellular transformation involved TP53, RB1 inactivation, RAS activation, and TERT-mediated telomere protection.
  • Transformation correlated with moderately enhanced levels of specific Pol III-transcribed RNAs (7SK, MRP, H1).
  • Several Pol III subunits and transcription factors (TFIIIB, TFIIIC, SNAPC1, POLR3G) showed increased mRNA and/or protein levels, with SNAPC1 and POLR3G strongly upregulated.

Conclusions:

  • Increased expression of Pol III transcription system components accompanies tumor formation.
  • A 2-fold increase in specific Pol III RNAs is sufficient to sustain tumor development.
  • These findings highlight the critical role of Pol III transcription in sustaining tumorigenesis.
Keywords:
7SK RNAPOLR3GPP2ARASRB1RNA polymerase IIITERTTP53defined transformationtranscription

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.4K
RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Published on: November 26, 2018

11.1K

Related Concept Videos

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.1K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.6K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.3K
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.2K
Transcription Initiation01:47

Transcription Initiation

Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
21.4K