Related Experiment Video

Updated: Feb 18, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

MicroRNA-1246 inhibits cell invasion and epithelial mesenchymal transition process by targeting CXCR4 in lung cancer

Xiaohui Xu, Lei Cao, Ye Zhang

    Cancer Biomarkers : Section a of Disease Markers
    |November 25, 2017
    PubMed
    Abstract

    Insights

    MicroRNA-1246 (miR-1246) is downregulated in lung cancer. Overexpression of miR-1246 inhibits cancer cell invasion and epithelial-mesenchymal transition by targeting CXCR4 and blocking key signaling pathways.

    Area of Science:

    • Molecular Oncology
    • Cell Biology
    • Cancer Research

    Background:

    • MicroRNAs (miRNAs) play a role in lung cancer development.
    • The specific function of miR-1246 in lung cancer remains unclear.

    Purpose of the Study:

    • To investigate the molecular mechanisms of miR-1246 in lung cancer.
    • To explore the role of miR-1246 in lung cancer cell invasion and epithelial-mesenchymal transition (EMT).

    Main Methods:

    • RT-qPCR to analyze miR-1246 expression in lung cancer cell lines.
    • Cell Counting Kit-8 (CCK-8), flow cytometry, and Transwell assays to assess cell viability, apoptosis, and invasion.
    • Luciferase reporter assay to identify miR-1246 targets.
    • Western blot to examine the JAK/STAT and PI3K/AKT signaling pathways.

    Main Results:

    • miR-1246 expression was significantly downregulated in lung cancer cell lines.
    • Overexpression of miR-1246 inhibited cell invasion and EMT markers (N-cadherin, Vimentin, ZEB1, Snail) while upregulating E-cadherin.
    • CXCR4 was identified as a direct target of miR-1246.
    • miR-1246 suppressed the JAK/STAT and PI3K/AKT signaling pathways via CXCR4 regulation.

    Conclusions:

    • miR-1246 acts as a tumor suppressor in lung cancer.
    • miR-1246 inhibits lung cancer cell invasion and EMT by targeting CXCR4.
    • miR-1246 exerts its effects by modulating the JAK/STAT and PI3K/AKT signaling pathways.
    Keywords:
    CXCR4cell invasionepithelial mesenchymal transitionlung cancermicroRNA-1246

    More Related Videos

    In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

    In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

    Published on: August 23, 2019

    7.8K
    Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

    Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

    Published on: April 6, 2012

    18.1K

    Related Concept Videos

    MicroRNAs01:22

    MicroRNAs

    MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
    24.3K
    MicroRNAs01:22

    MicroRNAs

    MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
    4.1K
    Metastasis02:30

    Metastasis

    Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
    Epithelial-to-Mesenchymal Transition
    The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
    6.7K
    Cancer Cell Migration through Invadopodia01:35

    Cancer Cell Migration through Invadopodia

    Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
    3.3K