Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering
Fan Shi1,2, Fuun Kawano2, Seon-Hye Emily Park2
1College of Precision Instrument and Optoelectronics Engineering, Tianjin University , Tianjin, China.
Abstract:
The organelle interface emerges as a dynamic platform for a variety of biological responses. However, their study has been limited by the lack of tools to manipulate their occurrence in live cells spatiotemporally. Here, we report the development of a genetically encoded light-inducible tethering (LIT) system allowing the induction of contacts between endoplasmic reticulum (ER) and mitochondria, taking advantage of a pair of light-dependent heterodimerization called an iLID system. We demonstrate that the iLID-based LIT approach enables control of ER-mitochondria tethering with high spatiotemporal precision in various cell types including primary neurons, which will facilitate the functional study of ER-mitochondrial contacts.
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