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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Updated: Feb 18, 2026

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
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New Developments in CRISPR/Cas-based Functional Genomics and their Implications for Research Using Zebrafish.

Sergey V Prykhozhij1, Lucia Caceres1, Jason N Berman1

  • 1Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.

Current Gene Therapy
|November 28, 2017
PubMed
Summary

CRISPR/Cas9 technology advances enable precise genome editing in zebrafish for disease modeling. New methods improve knock-ins and facilitate high-throughput genetic screens, enhancing preclinical research capabilities.

Keywords:
CRISPRCas9Genetic screenSingle-cell RNA sequencingZebrafishsgRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • Zebrafish Model Organisms

Background:

  • CRISPR/Cas9 technology has rapidly advanced in scope, versatility, and ease of use.
  • Zebrafish (Danio rerio) is a key vertebrate model for extensive CRISPR/Cas9 applications, particularly in disease modeling for preclinical testing of gene variants.
  • Advances in diverse species and systems offer opportunities to improve genome editing in zebrafish.

Purpose of the Study:

  • To review recent trends and technologies for enhancing genome editing precision and efficiency in zebrafish.
  • To explore applications of novel genome editing tools for temporal and spatial control in zebrafish.
  • To discuss improvements in oligo-based knock-ins and their relevance to zebrafish research.

Main Methods:

  • Application of artificial transcription factors for precise genome editing.
  • Utilizing drug-inducible or optogenetically-driven Cas9 expression for temporal and spatial control.
  • Employing chemically- or optogenetically-inducible dead Cas9 (dCas9) for gene regulation.
  • Leveraging oligonucleotides for precise knock-in modifications.
  • Implementing multiplexed sgRNA libraries with Cas9 for genetic screens.

Main Results:

  • New technologies enable temporal and spatial precision in zebrafish genome editing.
  • Oligonucleotide-mediated knock-ins allow for precise genomic modifications.
  • Multiplexed sgRNA libraries and Cas9 are poised to revolutionize genetic screens in zebrafish.
  • Methods for measuring single-cell phenotypes after mutagenic perturbation are advancing.

Conclusions:

  • Recent advances in genome editing technologies can significantly improve precision and efficiency in zebrafish.
  • Enhanced control over gene editing, including temporal and spatial regulation, is achievable.
  • Novel approaches using sgRNAs and advanced phenotyping methods will drive high-throughput genetic screens in zebrafish, advancing disease modeling and genetic research.