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Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

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Color-coded LED microscopy for quantitative phase imaging: Implementation and application to sperm motility analysis.

Wonchan Lee¹, Jun-Ho Choi², Suho Ryu¹

¹Yonsei University, School of Mechanical Engineering, Seoul 03722, Republic of Korea.

Methods (San Diego, Calif.)

|November 28, 2017

Summary

This summary is machine-generated.

Color-coded light-emitting diode (LED) microscopy (cLEDscope) offers multi-contrast and quantitative phase imaging. This novel technique enables real-time analysis, demonstrated here for sperm motility assessment.

Keywords:

Computational imaging Microscopy Phase retrieval Sperm analysis

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Area of Science:

Biomedical Optics
Computational Imaging
Microscopy

Background:

Quantitative phase imaging (QPI) provides label-free contrast for biological specimens.
Traditional QPI methods often require specialized hardware or complex setups.
Computational microscopy offers advanced imaging capabilities with enhanced flexibility.

Purpose of the Study:

To detail the implementation of color-coded light-emitting diode microscopy (cLEDscope) for quantitative phase imaging.
To present the imaging model and phase-retrieval algorithms for the cLEDscope system.
To demonstrate the application of cLEDscope for real-time sperm motility analysis.

Main Methods:

Utilized color-multiplexed illumination with specially designed LED patterns for simultaneous multi-contrast imaging.
Developed a computational algorithm to retrieve specimen phase distribution from single-exposure DPC images.
Derived an imaging model based on a weak-object transfer function for phase retrieval.

Main Results:

Successfully implemented cLEDscope for quantitative phase imaging of biological specimens.
Detailed procedures, features, and caveats of the cLEDscope setup and processing were outlined.
Demonstrated cLEDscope's utility as a sperm-motility analyzer through real-time quantitative imaging.

Conclusions:

cLEDscope is a versatile computational microscopy technique for multi-contrast and quantitative phase imaging.
The developed algorithms enable accurate phase retrieval from multiplexed illumination.
cLEDscope shows significant potential for quantitative biological analysis, such as sperm motility assessment.