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Engineering of biomolecules by bacteriophage directed evolution.

Andreas K Brödel1, Mark Isalan1, Alfonso Jaramillo2

  • 1Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.

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|November 28, 2017
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Summary

Phage-based selection systems enable in vivo directed evolution by linking biomolecule activity to bacteriophage (phage) growth. This powerful technique advances protein and RNA engineering for novel applications and understanding evolution.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Evolutionary Biology

Background:

  • Traditional in vivo directed evolution links biomolecule function to bacterial growth.
  • Recent advancements utilize bacteriophages (phages) for conditional growth-based selection.
  • Phages are viruses that infect and replicate within bacteria.

Purpose of the Study:

  • To review recent advancements in phage-based selection systems for in vivo directed evolution.
  • To highlight the diverse applications of these systems in protein and RNA engineering.

Main Methods:

  • Review of literature on phage-based selection systems for in vivo directed evolution.
  • Analysis of protein engineering applications, including transcription factors, polymerases, proteases, DNA-binding proteins, and protein-protein interactions.

Main Results:

  • Phage-based systems offer a novel approach to in vivo directed evolution.
  • These systems have been successfully applied to evolve a wide array of proteins.
  • Demonstrated utility in engineering transcription factors, polymerases, proteases, DNA-binding proteins, and protein-protein interactions.

Conclusions:

  • Phage-based selection significantly expands the toolkit for protein and RNA engineering.
  • Enables the development of biomolecules with tailored properties.
  • Provides deeper insights into fundamental evolutionary processes.