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Integrating protein engineering with process design for biocatalysis.

John M Woodley1

  • 1Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs Lyngby, Denmark jw@kt.dtu.dk.

Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
|November 28, 2017
PubMed
Summary
This summary is machine-generated.

Biocatalysis, using enzymes for sustainable chemical synthesis, is expanding beyond pharmaceuticals. This study introduces a new tool for protein engineering, guiding enzyme development based on process needs for improved industrial implementation.

Keywords:
biocatalysisbioprocess engineeringprocess intensificationprotein engineering

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Area of Science:

  • Biocatalysis and sustainable chemistry
  • Enzyme engineering and process development

Background:

  • Biocatalysis offers selective, safe, and sustainable chemical synthesis, with expanding applications beyond the pharmaceutical industry.
  • Traditionally, processes were adapted to enzyme properties, but protein engineering now allows enzyme optimization for specific industrial needs.
  • Integrating protein and process engineering is crucial for effective biocatalysis implementation, especially where production costs are a key driver.

Purpose of the Study:

  • To develop a tool that enhances the effectiveness of integrated protein and process engineering in biocatalysis.
  • To guide protein engineering efforts by setting targets based on specific process requirements.
  • To facilitate the broader industrial adoption of biocatalysis by improving enzyme suitability for diverse applications.

Main Methods:

  • Development of a novel tool for target-setting in biocatalysis.
  • Utilizing process requirements to direct protein engineering strategies.
  • Integrated approach combining enzyme and process development.

Main Results:

  • The developed tool effectively guides protein engineering by defining process-based targets.
  • Demonstrated improvement in the efficiency of developing tailored biocatalysts.
  • Facilitated a more streamlined approach to enzyme adaptation for industrial processes.

Conclusions:

  • The target-setting tool represents a significant advancement in optimizing biocatalysis for industrial applications.
  • This approach enhances the integration of protein engineering with process development, leading to more effective enzyme solutions.
  • The study contributes to advancing sustainable catalytic solutions for a rapidly changing industrial landscape.