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Related Concept Videos

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Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
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Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Related Experiment Video

Updated: Feb 18, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
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Biocontaining: Purification, Restoration, and Meaning-Making.

Helen Chapple, David Schenck

    Perspectives in Biology and Medicine
    |November 28, 2017
    PubMed
    Summary

    Biocontaining efforts during the Ebola outbreak provided social reassurance and meaning, despite limited impact on the pandemic. This study explores how biocontainment activities fostered societal comfort and understanding during the crisis.

    Area of Science:

    • Sociology of Medicine
    • Medical Anthropology
    • Public Health Communication

    Background:

    • The recent Ebola outbreak highlighted the social importance of biomedical interventions.
    • Biocontaining measures had minimal direct impact on pandemic spread.
    • Cultural theories by Douglas and Turner offer insights into meaning-making during crises.

    Purpose of the Study:

    • To analyze the role of biocontainment in social reassurance and meaning-making during the Ebola crisis.
    • To understand how biocontainment activities contributed to societal coping mechanisms.
    • To explore the cultural significance of biomedical practices in public health emergencies.

    Main Methods:

    • Fieldwork conducted at the Nebraska Biocontainment Unit (NBU).
    • Analysis of NBU educational materials.

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  • Follow-up interviews with NBU personnel.

    • Main Results:

    • Biocontaining activities served a crucial function in providing social reassurance.
    • The process of biocontainment facilitated meaning-making for U.S. and global society.
    • Cultural interpretations of biocontainment reinforced social significance beyond measurable pandemic effects.

    Conclusions:

    • Biocontainment's value extended beyond its epidemiological impact, serving vital social and cultural functions.
    • Understanding the cultural dimensions of public health interventions is essential during crises.
    • The study underscores the interplay between biomedical practices and societal meaning-making.