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In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
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In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

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Molecular Chaperone Receptors

Ayesha Murshid1, Jimmy Theriault1, Jianlin Gong2

  • 1Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, Boston, MA, 02215, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2017
PubMed

Insights

Extracellular heat shock proteins (HSP) bind to cell surface receptors, including c-type lectin receptors (CLR) and scavenger receptors (SR). This discovery advances understanding of HSP-mediated cell signaling and immunity.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Extracellular heat shock proteins (HSP) are crucial for cell signaling and immune responses.
  • Surface receptors on various cell types mediate many HSP effects.
  • Understanding these interactions is key to deciphering cellular communication.

Purpose of the Study:

  • To identify and characterize receptors that bind extracellular heat shock proteins (HSP).
  • To investigate the binding specificities of Hsp70 to different receptor classes.
  • To explore methods for discovering HSP receptors and studying their function.

Main Methods:

  • Utilized Chinese Hamster Ovary (CHO-K1) cells lacking endogenous HSP binding capacity.
  • Cloned candidate receptors into these null cells to assess HSP binding.
  • Employed techniques to study individual receptors and in vivo HSP receptor function.

Main Results:

  • Identified that Hsp70 binds strongly to at least two receptor classes: c-type lectin receptors (CLR) and scavenger receptors (SR).
  • Demonstrated Hsp70 binding to LOX-1 (a CLR and SR) via its c-type lectin binding domain (CTLD).
  • Showed Hsp70 binding to SR family members SREC-I and FEEL-1/CLEVER-1/STABILIN-1, which possess EGF-like repeats.

Conclusions:

  • Hsp70 interacts with multiple cell surface receptor families, including CLRs and SRs.
  • Specific domains like CTLD and extracellular repeats mediate these HSP-receptor interactions.
  • The study provides foundational methods for discovering and analyzing HSP receptors in vitro and in vivo.
Keywords:
ExtracellularHeatImmunityProteinReceptorScavengerShock

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