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A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
Thirunavukkarasu Annamalai1,2, Bokun Cheng3, Neelam Keswani1,2
1Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2017
Abstract:
Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
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