Related Experiment Video
Updated: Feb 18, 2026
14:16
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
Published on: February 11, 2009
19.7K
E-selectin gene in essential hypertension: a case-control study.
Kamna Srivastava1, Sudhir Chandra1, Rajiv Narang2
1Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India.
European Journal of Clinical Investigation
|November 28, 2017
Summary
The E-selectin Leu554Phe gene polymorphism is significantly associated with essential hypertension. Patients with hypertension show higher E-selectin gene expression at both mRNA and protein levels, suggesting a genetic link.
Area of Science:
- Genetics
- Cardiovascular Medicine
- Molecular Biology
Background:
- Hypertension is linked to endothelial cell dysfunction.
- E-selectin, an endothelial cell activation marker, has gene polymorphisms.
- The E-selectin Leu554Phe polymorphism's role in essential hypertension is understudied.
Purpose of the Study:
- To investigate the association between E-selectin gene Leu554Phe polymorphism and essential hypertension.
- To evaluate E-selectin gene expression levels in essential hypertension patients.
Main Methods:
- Genotyping of E-selectin Leu554Phe polymorphism using PCR-RFLP in 250 hypertensive patients and 250 controls.
- Quantification of E-selectin gene expression at mRNA (real-time PCR) and protein (Western blot) levels.
Main Results:
- A significant association was found between E-selectin genotypes (CT + TT) and essential hypertension (P < .0001).
- E-selectin mRNA expression was approximately 12-fold higher in hypertensive patients compared to controls.
- Elevated E-selectin protein levels (up to 1.9 times) were observed in patients with essential hypertension.
Conclusions:
- The E-selectin Leu554Phe gene polymorphism is significantly associated with essential hypertension.
- Increased E-selectin gene expression at mRNA and protein levels in patients suggests a genetic predisposition to essential hypertension.
More Related Videos
06:20Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure
Published on: August 23, 2011
16.5K
08:08Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta
Published on: June 10, 2015
11.9K
Related Concept Videos
01:25Selectins
4.4K
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
4.4K
01:30Hypertension III: Clinical Manifestations and Diagnostic Studies
632
Hypertension is asymptomatic and also referred to as the "silent killer" until it progresses to a severe stage or causes target organ disease. Patients may experience symptoms stemming from the strain on blood vessels and tissues in various organs or the heart's increased workload.Physical exams might show no abnormalities other than high blood pressure. Signs of vascular damage, when present, correspond to the organs supplied by the affected vessels, leading to target organ damage. For...
632
01:29Hypertension II: Pathophysiology
1.1K
Hypertension is a chronic condition in which the blood's force against artery walls is excessively high, posing risks such as heart disease. The condition's underlying mechanisms involve complex interactions among the cardiovascular, kidney, and autonomic nervous systems.Renin-Angiotensin-Aldosterone System (RAAS): This system significantly influences blood pressure regulation. When blood pressure decreases, the kidneys secrete renin. This enzyme transforms angiotensinogen, a plasma protein,...
1.1K
01:11Genome-wide Association Studies-GWAS
15.8K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
15.8K
01:18Hypertension and Regulation of Blood Pressure
4.5K
Hypertension, the most common cardiovascular disease, is diagnosed through repeated measurements of elevated blood pressure. Its risks, including damage to the kidney, heart, and brain, are directly proportional to blood pressure levels. Starting from 115/75 mm Hg, the risk of cardiovascular disease doubles with each increment of 20/10 mm Hg. The diagnosis relies on blood pressure measurements, not on patient symptoms, as hypertension is often asymptomatic until end-organ damage is imminent or...
4.5K
01:28Hypertension I: Introduction
986
Hypertension is a widespread, long-term medical condition where blood pressure in the arteries remains elevated. It is characterized by systolic blood pressure readings of 130 mm Hg or above or diastolic blood pressure (DBP) readings of 80 mm Hg or higher. Unmanaged hypertension poses significant health risks, making the distinction between primary (or essential) hypertension and secondary hypertension crucial, as their management and implications vary.Primary HypertensionPrimary hypertension,...
986