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Effects of riboflavin, calcium-phosphate layer and adhesive system on stress-strain behavior of demineralized dentin
Tissiana Bortolotto1, Anastasia Ryabova1, Izabella Nerushay1
1Division of Cariology and Endodontology, of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Purpose:
To evaluate if three dentin treatments improved mechanical properties of demineralized dentin.
Methods:
Dentin slices were demineralized and treated with a universal adhesive, Scotchbond Universal (SBU), a cross-linker, Riboflavin (RF), and a calcium phosphate-based product, Teethmate (TM). The groups (n= 8 per group) were: Group 1: SBU, Group 2: RF + SBU, Group 3: RF + TM + SBU. Tensile tests were performed; stress/strain curves and E modulus were calculated. Differences between groups were assessed by one-way ANOVA and Duncan post hoc test.
Results:
At high strains, no significant differences in E moduli were observed between dentin specimens treated only with SBU and those treated with RF + SBU. A significantly higher E modulus was observed in dentin specimens treated with RF + TM + SBU. In the presence of an adhesive system, crosslinking collagen with RF and TM addition significantly improved mechanical properties of dentin.
Clinical Significance:
Restitution of mineral content into dentin, in addition to collagen strengthening, may significantly improve mechanical properties of previously demineralized dentin, when covered by an adhesive system in a reasonable clinical timeframe.
