Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

733
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
733
Transposons01:24

Transposons

2.1K
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
2.1K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.9K
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

19.4K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
19.4K
DNA-only Transposons02:57

DNA-only Transposons

17.6K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metagenomic Evaluation of Oral Microbiota in Patients Affected by Oral Lichen Planus: A Pilot Study.

Oral diseases·2026
Same author

Genomic insights into the population structure and adaptive variation of Mullus barbatus in the Mediterranean Sea.

BMC ecology and evolution·2025
Same author

Unlocking the potential: m6A-RNA methylation in severe epidermolysis bullosa simplex.

Bioscience reports·2025
Same author

A chromosome-level genome assembly of the European green toad (Bufotes viridis).

G3 (Bethesda, Md.)·2025
Same author

Author Correction: Life history and ancestry of the late Upper Palaeolithic infant from Grotta delle Mura, Italy.

Nature communications·2024
Same author

Life history and ancestry of the late Upper Palaeolithic infant from Grotta delle Mura, Italy.

Nature communications·2024
Same journal

Peripheral B-cell receptor repertoire predicts immune-related adverse events following immune checkpoint inhibitor therapy in advanced renal cell carcinoma.

Scientific reports·2026
Same journal

Effects of black soldier fly (Hermetia illucens L.) larvae zoocompost on the mineral element content of blue honeysuckle berries.

Scientific reports·2026
Same journal

Investigation on absorption refrigeration performance of R1243zf with imidazolium ionic liquid as the working pairs.

Scientific reports·2026
Same journal

DeepTriage-CN: integrating clinical text with vital signs for emergency department admission prediction in an aging population.

Scientific reports·2026
Same journal

Gold nanoparticles as dual-action antiviral agents: disruption of SARS-CoV-2 viral envelopes and RNA integrity.

Scientific reports·2026
Same journal

Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Feb 18, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.4K

NumtS colonization in mammalian genomes.

F M Calabrese1, D L Balacco2, R Preste3

  • 1Department of Biology, University of Bari, Bari, 70124, Italy.

Scientific Reports
|November 29, 2017
PubMed
Summary
This summary is machine-generated.

Mitochondrial DNA (NumtS) integration into nuclear genomes varies across species, with hotspots identified. Early colonization events in mammals predate major divergences, influencing genomic evolution.

More Related Videos

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
09:55

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Published on: August 12, 2021

4.3K
An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

4.0K

Related Experiment Videos

Last Updated: Feb 18, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.4K
Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
09:55

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Published on: August 12, 2021

4.3K
An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

4.0K

Area of Science:

  • Genomics
  • Evolutionary Biology
  • Molecular Biology

Background:

  • Mitochondrial DNA (mtDNA) integration into nuclear genomes (NumtS) is a dynamic evolutionary process contributing to genomic variability in eukaryotes.
  • Despite extensive genomic sequencing, the distribution and evolutionary history of NumtS remain incompletely characterized in most species.
  • Understanding NumtS dynamics is crucial for insights into genome evolution and inter-species genomic comparisons.

Purpose of the Study:

  • To investigate the genomic distribution and evolutionary patterns of NumtS across 23 eukaryotic species.
  • To determine the timing and mechanisms of NumtS colonization, particularly within mammalian lineages.
  • To identify potential hotspots for mtDNA integration into nuclear genomes.

Main Methods:

  • In silico analysis using cross-species similarity searches to identify NumtS across 23 eukaryotic genomes.
  • Intra- and interspecies comparative genomics to map NumtS distribution.
  • Phylogenetic analyses of NumtS from specific mitochondrial DNA loci in mammals.

Main Results:

  • Identified specific mitochondrial DNA regions as hotspots for nuclear genome colonization.
  • Detected a significant abundance of NumtS in platypus and opossum, suggesting ancient colonization events predating mammal divergence (160-210 million years ago).
  • Phylogenetic analyses revealed distinct NumtS evolutionary trajectories in primate versus non-primate mammal genomes.

Conclusions:

  • NumtS distribution is shaped by both ancient colonization events and ongoing species-specific genomic dynamics.
  • The study provides evidence for early and persistent mtDNA integration in mammalian nuclear genomes.
  • Differential evolution of NumtS in primates and non-primates highlights lineage-specific genomic adaptation processes.