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Reconstruct Human Retinoblastoma In Vitro
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Expression of multidrug resistance proteins in retinoblastoma

Swati Shukla1, Arpna Srivastava1, Sunil Kumar1

  • 1Department of Ocular Biochemistry, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi 110029, India.

International Journal of Ophthalmology
|November 29, 2017
PubMed
Abstract

Insights

Multidrug resistant proteins are inherently present in retinoblastoma, contributing to chemotherapy treatment failure. This study investigated these resistance mechanisms in retinoblastoma cells and primary cultures.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Retinoblastoma (RB) is a pediatric eye cancer.
  • Chemotherapy is a primary treatment for RB.
  • Multidrug resistance (MDR) poses a significant challenge in RB treatment.

Purpose of the Study:

  • To investigate the mechanisms of multidrug resistance in retinoblastoma.
  • To understand in vivo drug resistance in retinoblastoma.
  • To identify key proteins involved in RB drug resistance.

Main Methods:

  • Generation of drug-resistant Y79 human RB cell lines (vincristine, etoposide, carboplatin).
  • Preparation of primary cultures from enucleated eyes after chemotherapy (PCNC).
  • Assessment of chemosensitivity using MTT assay and Western blot analysis for protein expression (p53, Bcl-2, P-gp, Mrp-1, Lrp).

Main Results:

  • Drug-resistant Y79 cells and PCNC exhibited reduced chemosensitivity.
  • Increased Bcl-2 expression was observed in resistant cells and PCNC.
  • Elevated P-glycoprotein (P-gp) and Multidrug resistance protein 1 (Mrp-1) expression were noted in resistant Y79 cells and PCNC.

Conclusions:

  • Multidrug resistant proteins are intrinsically present in retinoblastoma.
  • These proteins contribute to treatment failure in retinoblastoma management.
  • Targeting these MDR proteins may improve chemotherapy efficacy in RB.
Keywords:
chemotherapymultidrug resistancemultidrug resistance associated proteinsretinoblastoma

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