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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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From molecular engineering to process engineering: development of high-throughput screening methods in enzyme

Lidan Ye1, Chengcheng Yang1, Hongwei Yu2

  • 1Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, People's Republic of China.

Applied Microbiology and Biotechnology
|November 29, 2017
PubMed
Summary
This summary is machine-generated.

Directed evolution of enzymes uses high-throughput screening (HTS) methods to improve biocatalysis for sustainable development. Recent advances integrate directed evolution with metabolic engineering, shifting focus from molecular to process engineering.

Keywords:
BiocatalysisBiosynthesisCategorizationDirected evolutionHigh-throughput screening method

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A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
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Area of Science:

  • Biocatalysis and enzyme engineering
  • Sustainable chemistry
  • Metabolic engineering

Background:

  • Enzymes offer sustainable alternatives to traditional chemical processes due to their mild reaction conditions, high activity, and selectivity.
  • Natural enzymes often exhibit limitations in activity or enantioselectivity with non-natural substrates, hindering industrial applications.
  • Directed evolution overcomes these limitations by improving enzyme properties without requiring detailed mechanistic understanding.

Purpose of the Study:

  • To provide a comprehensive overview of high-throughput screening (HTS) methods for enzyme directed evolution.
  • To discuss assay design strategies and present typical examples of HTS methods.
  • To explore the integration of directed evolution with metabolic engineering for process optimization.

Main Methods:

  • Review and categorization of recent HTS methods based on reaction principles.
  • Summary of in vitro and in vivo screening techniques, including indicator addition and multi-enzyme systems.
  • Discussion of computational and machine-assisted screening approaches.

Main Results:

  • A wide array of HTS methods have been developed for rapid evaluation of enzyme mutant libraries.
  • Recent trends show a move towards integrating directed evolution with metabolic engineering, utilizing metabolites as HTS indicators.
  • This integration signifies a shift from molecular engineering to process engineering in biocatalysis.

Conclusions:

  • Effective HTS methods are crucial for successful enzyme directed evolution.
  • The evolution of HTS methodologies is expanding their applicability in industrial biocatalysis.
  • Future directions involve further integration with metabolic engineering and process development for enhanced biocatalytic solutions.