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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Probe-Substrate Distance Control in Desorption Electrospray Ionization.

Tyler J Yarger1, Elizabeth M Yuill1, Lane A Baker2

  • 1Department of Chemistry, Indiana University, 800 E. Kirkwood Ave., Bloomington, IN, 47405, USA.

Journal of the American Society for Mass Spectrometry
|November 29, 2017
PubMed
Summary
This summary is machine-generated.

We developed probe-substrate distance control for desorption electrospray ionization (DESI). This technique optimizes spray current for improved mass spectrometry imaging and future applications.

Keywords:
DESIImagingMSISurface

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Surface Science

Background:

  • Desorption electrospray ionization (DESI) is a versatile ambient ionization technique.
  • Precise control over the probe-substrate distance (Dps) is crucial for optimizing DESI performance.
  • Understanding the relationship between Dps and spray characteristics is essential for method development.

Purpose of the Study:

  • To introduce and investigate probe-substrate distance (Dps) control in DESI.
  • To systematically examine the impact of Dps on key experimental parameters like voltage, flow rate, and nebulizing gas pressure.
  • To explore the utility of spray current as a feedback signal for positioning in DESI.

Main Methods:

  • Implementation of a novel Dps control system for DESI.
  • Systematic variation of Dps while monitoring spray current.
  • Simultaneous mass spectrometry imaging (MSI) with Dps-controlled DESI.
  • Investigation of voltage, flow rate, and nebulizing gas pressure effects on spray current at varying Dps.

Main Results:

  • A critical Dps was identified where spray current behavior inverts.
  • Decreasing Dps initially increases distance-dependent spray current until a critical point.
  • Beyond the critical point, further Dps reduction decreases spray current due to gas flow constriction.
  • Spray current was successfully utilized as a feedback signal for automated positioning.

Conclusions:

  • Dps control offers a new paradigm for optimizing DESI performance.
  • The identified critical Dps and spray current feedback mechanism enhance MSI capabilities.
  • This technique holds significant potential for clinical diagnostics, biological investigations, and materials characterization through structure-function relationship studies.