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Rapid re-identification of human samples using portable DNA sequencing.

Sophie Zaaijer1,2, Assaf Gordon2, Daniel Speyer1,2

  • 1Department of Computer Science, New York Genome Center, New York, United States.

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|November 29, 2017
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Summary

MinION sketching offers rapid, inexpensive DNA re-identification using few single nucleotide polymorphisms (SNPs). This method enables near real-time applications for cell line and tissue authentication, improving research reproducibility.

Keywords:
DNA fingerprintingcell biologycell line authenticationevolutionary biologyforensicsgenomicshumannanopore sequencingre-identification

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • DNA re-identification is crucial for applications like cell line authentication and forensics.
  • Current DNA re-identification methods face challenges with high latency and restricted access.

Purpose of the Study:

  • To introduce 'MinION sketching,' a novel, rapid, and portable strategy for human DNA re-identification.
  • To enable near real-time DNA re-identification applications.

Main Methods:

  • Utilizes the MinION sequencing platform for rapid DNA analysis.
  • Requires minimal sequencing time (as few as 3 minutes) and a small number of random single nucleotide polymorphisms (SNPs) (60-300).
  • Leverages the increasing availability of genomic reference data.

Main Results:

  • Achieves robust human DNA re-identification with high speed and low cost.
  • Demonstrates the potential for near real-time DNA re-identification.
  • Enables efficient cell line and tissue authentication in research and clinical settings.

Conclusions:

  • MinION sketching provides a significantly faster and more reliable alternative to current cell line authentication practices.
  • This method can help mitigate research irreproducibility issues stemming from cell culture mix-ups and contamination.
  • Facilitates periodic authentication of cell lines and tissues in biobanks and research.