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Related Experiment Video

Updated: Feb 18, 2026

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

A sterilization method for decellularized xenogeneic cardiovascular scaffolds.

Cátia Fidalgo¹, Laura Iop¹, Manuela Sciro²

¹Department of Cardiac, Thoracic and Vascular Sciences, University of Padua, Padua, Italy; Venetian Institute of Molecular Medicine, Padua, Italy.

Acta Biomaterialia

|November 30, 2017

Summary

This summary is machine-generated.

A new two-step sterilization method using antibiotics/antimycotics and peracetic acid effectively sterilizes decellularized pericardial scaffolds, preserving tissue integrity for cardiovascular tissue engineering applications.

Keywords:

Decellularization Peracetic acid Pericardium Sterilization methodology Xenogeneic scaffolds

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Last Updated: Feb 18, 2026

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Area of Science:

Biomaterials Science
Tissue Engineering
Sterilization Technologies

Background:

Decellularized xenogeneic scaffolds are promising for cardiovascular tissue engineering.
Lack of effective sterilization methods hinders clinical translation of these biomaterials.

Purpose of the Study:

To investigate an effective two-step sterilization method for decellularized porcine and bovine pericardium.
To develop and validate a sterilization assessment protocol for decellularized scaffolds.

Main Methods:

A two-step sterilization process using an antibiotics/antimycotic cocktail followed by peracetic acid.
A sterilization assessment protocol involving controlled contamination, sterility testing, and contaminant identification/quantification (MALDI-TOF, MPN).

Main Results:

The antibiotics/antimycotic + peracetic acid method successfully sterilized both porcine and bovine decellularized pericardium.
The sterilization process preserved structural integrity, biocompatibility, and biomechanical properties.
Minor impairment in surface properties and cellular adhesion was observed in porcine pericardium.

Conclusions:

The developed sterilization method is suitable for decellularized pericardial scaffolds.
The sterilization assessment protocol effectively monitors and optimizes decontamination methods.
This approach facilitates the clinical translation of decellularized xenogeneic cardiovascular substitutes.