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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Nucleosome Remodeling02:54

Nucleosome Remodeling

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Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Related Experiment Video

Updated: Feb 18, 2026

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

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Cardiac-enriched BAF chromatin-remodeling complex subunit Baf60c regulates gene expression programs essential for

Xin Sun1,2, Swetansu K Hota3,4, Yu-Qing Zhou5

  • 1Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, M5G 1X8 Canada.

Biology Open
|November 30, 2017
PubMed
Summary

Baf60c is crucial for heart development, regulating genes essential for cardiomyocyte function. Its loss causes cardiac defects, highlighting its role in coordinating cardiac gene expression programs.

Keywords:
Chromatin remodelingEmbryoGene regulationHeart

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Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
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Area of Science:

  • Molecular Biology
  • Cardiovascular Biology
  • Epigenetics

Background:

  • Chromatin-remodeling complexes control organ-specific properties, but their mechanisms remain unclear.
  • The cardiac-enriched BAF chromatin complex subunit, Baf60c (Smarcd3), has an incompletely understood role in heart development.

Purpose of the Study:

  • To elucidate the function of Baf60c in cardiac development and function.
  • To identify the molecular mechanisms by which Baf60c regulates cardiac gene expression.

Main Methods:

  • Constitutive and conditional gene deletion of Baf60c in mouse models.
  • Analysis of cardiac morphology, function, and gene expression.
  • Yeast two-hybrid screening and co-immunoprecipitation to assess protein interactions.

Main Results:

  • Loss of Baf60c resulted in embryonic cardiac hypoplasia and severe cardiac dysfunction.
  • Conditional deletion in cardiomyocytes led to postnatal dilated cardiomyopathy and impaired contractility.
  • Baf60c regulates genes involved in cardiac contractility, sarcomere function, and metabolism.
  • Baf60c directly interacts with Myocardin (MYOCD), a key transcriptional co-factor.

Conclusions:

  • Baf60c is essential for normal heart development and function.
  • Baf60c coordinates a critical gene expression program for cardiomyocyte functional properties.
  • The interaction between Baf60c and MYOCD is vital for regulating cardiac-specific gene networks.