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An in vitro radiolabel uptake viability assay for Onchocerca microfilariae
H L Callahan1, J M Wakeman, R K Crouch
1Department of Ophthalmology, Medical University of South Carolina, Charleston 29425.
The Journal of Parasitology
|February 1, 1989
Abstract:
A radiolabel uptake viability assay for Onchocerca cervicalis using [3H]2-deoxy-D-glucose in Hanks' balanced salt solution, pH 7.5, at 30 C is described and compared to the traditional visual motility assay. A correlation of r = 0.92 between the assays was found, with the radiolabel uptake method apparently a more sensitive indicator of microfilarial viability.