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Updated: Feb 18, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Bovine origin Staphylococcus aureus: A new zoonotic agent?
Relangi Tulasi Rao1, Kannan Jayakumar1, Pavitra Kumar2
1Department of Animal Behaviour & Physiology, School of Biological Sciences, Madurai Kamaraj University, Madurai - 625 021, Tamil Nadu, India.
Bovine Staphylococcus aureus strains are more virulent and pose a higher zoonotic risk than ovine strains. Healthy cattle can serve as reservoirs for this pathogen, highlighting the need for further investigation into bovine-origin S. aureus.
Area of Science:
- Veterinary Microbiology
- Zoonotic Diseases
- Bacterial Pathogenesis
Background:
- Staphylococcus aureus is a significant pathogen with zoonotic potential.
- Understanding the differences in virulence and host adaptation between animal-derived strains is crucial for public health.
- Bovine and ovine origins represent important reservoirs for S. aureus.
Purpose of the Study:
- To characterize Staphylococcus aureus strains from bovine and ovine origins.
- To compare the virulence of S. aureus between bovine and ovine hosts.
- To assess the zoonotic importance of animal-origin S. aureus.
Main Methods:
- Molecular characterization using conventional polymerase chain reaction (PCR).
- Virulence assessment employing a chick embryo model.
- Statistical analysis performed using R software.
Main Results:
- Prevalence of S. aureus was 42.62% in bovine and 28.35% in ovine samples.
- Methicillin-resistant S. aureus (MRSA) prevalence was low (6.8%) in both hosts.
- Bovine-origin S. aureus strains demonstrated significantly higher virulence (p=0.02795) compared to ovine strains.
Conclusions:
- Healthy bovines act as reservoirs for Staphylococcus aureus.
- Bovine-origin S. aureus strains exhibit greater virulence and a higher probability of zoonotic transmission.
- Further gene knockout studies are recommended to confirm the zoonotic potential of bovine strains.
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