A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation...

Area of Science:

• Biotechnology
• Pharmacology
• Microfluidics

Background:

• Microphysiological systems (MPS) are crucial for advanced drug screening.
• Current MPS platforms often lack throughput and comprehensive multi-organ integration.
• Developing scalable and user-friendly MPS is essential for preclinical research.

Purpose of the Study:

• To develop a novel multi-throughput, multi-organ-on-a-chip system.
• To establish a pneumatic pressure-driven platform for parallelized organoid experiments.
• To enable pipette-friendly liquid handling for diverse cell culture assays.

Main Methods:

• Construction of an eight-throughput two-organ system and a four-throughput four-organ system on a microplate-sized platform.
• Utilizing pneumatic pressure for medium circulation and parallelized culture.
• Performing cell seeding, staining, growth analysis, gene expression, and LC-MS analysis.

Main Results:

• Demonstrated the anti-cancer effect of capecitabine (CAP) metabolite 5-fluorouracil (5-FU) on liver-cancer models.
• Evaluated the efficacy of 5-FU and its prodrugs (CAP, tegafur) on multi-organ models including cancer and connective tissue.
• Confirmed the system's capability for drug metabolism and toxicity assessments.

Conclusions:

• The developed pneumatic pressure-driven multi-organ-on-a-chip system offers high throughput and flexibility.
• This MPS platform effectively models drug pharmacokinetics and pharmacodynamics across multiple organs.
• The system provides a valuable tool for preclinical drug evaluation and personalized medicine.