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Design and Application of a DNA-Encoded Macrocyclic Peptide Library
Zhengrong Zhu1, Alex Shaginian1, LaShadric C Grady1
1GlaxoSmithKline , 200 Cambridge Park Dr., Cambridge, Massachusetts 02140, United States.
ACS Chemical Biology
|November 30, 2017
Abstract:
A DNA-encoded macrocyclic peptide library was designed and synthesized with 2.4 × 1012 members composed of 4-20 natural and non-natural amino acids. Affinity-based selection was performed against two therapeutic targets, VHL and RSV N protein. On the basis of selection data, some peptides were selected for resynthesis without a DNA tag, and their activity was confirmed.
