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Updated: Feb 17, 2026

Bridging the Bio-Electronic Interface with Biofabrication
Published on: June 6, 2012
Ca2+-Switchable Glucose Dehydrogenase Associated with Electrochemical/Electronic Interfaces: Applications to
Ashkan Koushanpour1, Maria Gamella1, Zhong Guo2
1Department of Chemistry and Biomolecular Science, Clarkson University , Potsdam, New York 13699-5810, United States.
Abstract:
An artificial Ca2+-regulated PQQ glucose dehydrogenase (PQQ-GDH) enzyme was electrically connected to conducting electrodes and semiconductor interfaces. Direct electron transfer from the enzyme to the conducting electrode support was stimulated by the addition of Ca2+ cations resulting in reversible enzyme activation. A signal-switchable biofuel cell and biomolecular release have been realized using the Ca2+-activated enzyme immobilized on conducting electrodes. Interfacing the signal-switchable enzyme with a semiconductor chip allowed electronic read out of the enzyme ON-OFF states. The developed approach based on the signal-regulated PQQ-GDH enables numerous bioelectrochemical/bioelectronic applications of the developed systems in signal-activated biosensors and biofuel cells, as well as in biomolecular computing/logic systems.
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