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Cell-cycle-specific Cellular Responses to Sonoporation.
Pengfei Fan1, Yi Zhang1, Xiasheng Guo1
1Key Laboratory of Modern Acoustics (Nanjing University), Ministry of Education, Nanjing University, Nanjing 210093, China.
Theranostics
|December 1, 2017
Summary
Microbubble sonoporation enhances gene/drug delivery. S-phase cells are optimal for this cancer therapy due to increased permeability and faster cytoskeleton disassembly.
Area of Science:
- Biophysics
- Cell Biology
- Nanotechnology
Background:
- Microbubble-mediated sonoporation facilitates intracellular delivery of therapeutic agents.
- The biophysical mechanisms of microbubble-cell interactions and the impact of cell cycle phase remain unclear.
- Understanding these mechanisms is crucial for optimizing gene/drug delivery.
Purpose of the Study:
- To investigate the influence of cell cycle phase on cellular responses to microbubble sonoporation.
- To elucidate the impact on cell membrane and cytoskeleton.
- To identify optimal cell cycle phases for enhanced therapeutic agent delivery.
Main Methods:
- Human cervical epithelial carcinoma (HeLa) cells were synchronized into individual cell cycle phases.
- Atomic force microscopy (AFM) was used to assess cell topography and stiffness.
- A real-time fluorescence imaging system analyzed cell membrane permeabilization and cytoskeleton arrangement post-sonoporation.
Main Results:
- G1-phase cells exhibited greater height and elastic modulus compared to other phases.
- S-phase cells were the flattest and softest, showing the greatest enhancement in membrane permeability.
- S-phase cells demonstrated the fastest cytoskeleton disassembly following sonoporation.
Conclusions:
- S-phase represents a preferred cell cycle phase for microbubble sonoporation treatment.
- This finding supports targeted gene/drug delivery strategies in cancer therapy.
- Optimizing sonoporation based on cell cycle phase can improve therapeutic efficacy.
Keywords:
Microbubble-mediated sonoporationbubble-cell-interactioncell cyclecytoskeleton disassemblymembrane permeabilizationMore Related Videos
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