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Cell-cycle-specific Cellular Responses to Sonoporation.

Pengfei Fan1, Yi Zhang1, Xiasheng Guo1

  • 1Key Laboratory of Modern Acoustics (Nanjing University), Ministry of Education, Nanjing University, Nanjing 210093, China.

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|December 1, 2017
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Summary
This summary is machine-generated.

Microbubble sonoporation enhances gene/drug delivery. S-phase cells are optimal for this cancer therapy due to increased permeability and faster cytoskeleton disassembly.

Keywords:
Microbubble-mediated sonoporationbubble-cell-interactioncell cyclecytoskeleton disassemblymembrane permeabilization

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Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Microbubble-mediated sonoporation facilitates intracellular delivery of therapeutic agents.
  • The biophysical mechanisms of microbubble-cell interactions and the impact of cell cycle phase remain unclear.
  • Understanding these mechanisms is crucial for optimizing gene/drug delivery.

Purpose of the Study:

  • To investigate the influence of cell cycle phase on cellular responses to microbubble sonoporation.
  • To elucidate the impact on cell membrane and cytoskeleton.
  • To identify optimal cell cycle phases for enhanced therapeutic agent delivery.

Main Methods:

  • Human cervical epithelial carcinoma (HeLa) cells were synchronized into individual cell cycle phases.
  • Atomic force microscopy (AFM) was used to assess cell topography and stiffness.
  • A real-time fluorescence imaging system analyzed cell membrane permeabilization and cytoskeleton arrangement post-sonoporation.

Main Results:

  • G1-phase cells exhibited greater height and elastic modulus compared to other phases.
  • S-phase cells were the flattest and softest, showing the greatest enhancement in membrane permeability.
  • S-phase cells demonstrated the fastest cytoskeleton disassembly following sonoporation.

Conclusions:

  • S-phase represents a preferred cell cycle phase for microbubble sonoporation treatment.
  • This finding supports targeted gene/drug delivery strategies in cancer therapy.
  • Optimizing sonoporation based on cell cycle phase can improve therapeutic efficacy.