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Updated: Feb 17, 2026
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
Published on: March 7, 2012
In vivo study of optical speckle decorrelation time across depths in the mouse brain
Muhammad Mohsin Qureshi1,2, Joshua Brake3,2, Hee-Jae Jeon1
1Department of Biomedical Science and Engineering, Institute of Integrated Technology (IIT), Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-Ro, Buk-gu, Gwangju 61005, South Korea.
Abstract:
The strong optical scattering of biological tissue confounds our ability to focus light deeply into the brain beyond depths of a few hundred microns. This challenge can be potentially overcome by exploiting wavefront shaping techniques which allow light to be focused through or inside scattering media. However, these techniques require the scattering medium to be static, as changes in the arrangement of the scatterers between the wavefront recording and playback steps reduce the fidelity of the focus that is formed. Furthermore, as the thickness of the scattering medium increases, the influence of the dynamic nature becomes more severe due to the growing number of scattering events experienced by each photon. In this paper, by examining the scattering dynamics in the mouse brain in vivo via multispeckle diffusing wave spectroscopy (MSDWS) using a custom fiber probe that simulates a point-like source within the brain, we investigate the relationship between this decorrelation time and the depth of the point-like light source inside the living mouse brain at depths up to 3.2 mm.
