Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methods for depleting brain glutathione.

T Masukawa1, M Sai, Y Tochino

  • 1Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.

Life Sciences
|January 1, 1989
PubMed
Summary

Researchers identified cyclohexene-1-one and cycloheptene-1-one as effective compounds for depleting brain reduced glutathione (GSH) in mice. These substances offer new methods for studying GSH

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictors for onset of extended-spectrum beta-lactamase-producing Escherichia coli-induced bacteraemia: a systematic review and meta-analysis.

The Journal of hospital infection·2023
Same author

Thoracic duct and cisterna chyli: evaluation with multidetector row CT.

The British journal of radiology·2012
Same author

Nitric oxide and related enzymes in asthma: relation to severity, enzyme function and inflammation.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2011
Same author

Increased levels of angiopoietin-2 in induced sputum from smoking asthmatic patients.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2009
Same author

Correlations of health-related quality of life questionnaire results in asthma and allergic rhinitis: effects of a leukotriene receptor antagonist.

The Journal of international medical research·2008
Same author

[Results of surgical treatment for thymic epithelial tumors with special reference to the WHO classification].

Kyobu geka. The Japanese journal of thoracic surgery·2002

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pharmacology

Background:

  • Reduced glutathione (GSH) plays crucial roles in brain function.
  • Understanding methods to modulate brain GSH levels is vital for research.
  • Existing techniques for brain GSH depletion are limited.

Purpose of the Study:

  • To identify compounds capable of effectively depleting reduced glutathione (GSH) in the brain.
  • To evaluate the efficacy of various chemical agents on cerebral GSH levels in vivo.
  • To establish novel methods for manipulating brain GSH for further research.

Main Methods:

  • Systemic administration (intraperitoneal injection) of various compounds to mice.
  • Direct central administration (intracerebroventricular injection) of selected compounds.
  • Quantification of brain and liver GSH levels at different time points post-administration.

Main Results:

  • Cyclohexene-1-one, cycloheptene-1-one, and diethyl maleate significantly depleted brain GSH (40-60%) after systemic administration.
  • Intracerebroventricular injection of cyclohexene-1-one and cycloheptene-1-one caused substantial brain GSH depletion (60-80%) with prolonged effects.
  • Some compounds showed mild depletion, while buthionine sulfoximine had no effect on cerebral GSH.

Conclusions:

  • Cyclohexene-1-one and cycloheptene-1-one are potent agents for depleting brain GSH via both systemic and direct central administration.
  • These compounds' water-solubility facilitates cerebral penetration.
  • The identified compounds provide valuable tools for investigating the functional roles of cerebral GSH in vivo.

Related Experiment Videos