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A comprehensive evaluation of constraining amino acid biosynthesis in compartmented models for metabolic flux

Mathias Lehnen1, Birgitta E Ebert1, Lars M Blank1

  • 1iAMB - Institute of Applied Microbiology, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringer Weg 1, D-52074 Aachen, Germany.

Metabolic Engineering Communications
|December 1, 2017
PubMed

Keywords:
13C-metabolic flux analysisACCOA, acetyl-CoACompartmented metabolismEukaryotesGLY, glycineH. polymorphaILE, isoleucineLEU, leucineMDV, mass distribution vectorMFA, metabolic flux analysisNon-conventional yeastPYR, pyruvateS. cerevisiaeSER, serineSd, flux solution from a fully constrained modelSdmin, flux solution from a model with minimal constraintsSf, flux solution from an unconstrained modelTHR, threonineTP, TargetP 1.1WP, WoLF PSORT

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