Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ophthalmic Drug Delivery Systems

Ophthalmic Drug Delivery Systems

Ophthalmic drug delivery faces major limitations due to poor absorption across the corneal membrane. This process is primarily driven by diffusion and is influenced by two main factors: the physicochemical properties of the drug and tear drainage. Most ophthalmic drugs, such as pilocarpine, epinephrine, atropine, and local anesthetics, are weak bases. They are typically formulated at an acidic pH to enhance chemical stability. However, this leads to high ionization, reducing their ability to...

Drug Delivery: Miscellaneous Routes

Drug Delivery: Miscellaneous Routes

Drug delivery methods like oral inhalation, nasal sprays, transdermal patches, eye drops, intravitreal injection, and rectal administration provide localized effects with reduced toxicity.
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs...

Open Angle Glaucoma: Treatment

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...

Angle Closure Glaucoma: Treatment

Angle Closure Glaucoma: Treatment

Angle-closure glaucoma, or closed-angle glaucoma, is an eye condition where the iris bulges out and blocks the iridocorneal angle, resulting in a buildup of aqueous humor and increased intraocular pressure. Immediate medical attention is necessary due to the sudden onset of symptoms. The treatment for angle-closure glaucoma includes short-term and long-term approaches. Short-term treatment involves using eye drops like pilocarpine to lower intraocular pressure by increasing aqueous humor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ocular delivery of different valosin-containing protein (VCP) inhibitory formulations prevents retinal degeneration in rho<sup>∆I255</sup> mice.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

PARP Activity Is Essential for Retinal Photoreceptor Survival in the Human Homologous Rho<sup>I255del</sup> Mouse Model for Autosomal Dominant Retinitis Pigmentosa.

Journal of neurochemistry·2025

Same author

Quantitative Proteomics Identifies Potential Molecular Adaptations in Mouse Models of Congenital Stationary Night Blindness Type 2.

Molecular & cellular proteomics : MCP·2025

Same author

APOSTEL-R Recommendations for Reporting Retinal Optical Coherence Tomography Studies in Rodents.

Neurology(R) neuroimmunology & neuroinflammation·2025

Same author

Exploration of the visual streak of the Mongolian gerbil as a model for the human central retina.

Frontiers in medicine·2025

Same author

Autogene cevumeran with or without atezolizumab in advanced solid tumors: a phase 1 trial.

Nature medicine·2025

Search research articles

Related Experiment Video

Updated: Feb 17, 2026

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Published on: May 23, 2025

Advanced Ocular Injection Techniques for Therapy Approaches.

Regine Mühlfriedel¹, Marina Garcia Garrido², Christine Wallrapp³

¹Division of Ocular Neurodegeneration, Centre for Ophthalmology, Institute for Ophthalmic Research, University of Tübingen, Elfriede-Aulhorn-Str.7, 72076, Tübingen, Germany. regine.muehlfriedel@uni-tuebingen.de.

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2017

Summary

This summary is machine-generated.

This study details a method for delivering therapeutics to small eyes via transscleral pars plana intravitreal transfer using stem cells. This technique allows for precise delivery and tracking of therapeutic molecules for inherited eye diseases.

Keywords:

In vivo imaging Intraocular delivery Intravitreal implantation Micro CellBeads®Mouse eye Photoreceptor cells Retina Transscleral pars plana injection

More Related Videos

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Published on: March 16, 2022

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Published on: May 31, 2024

Related Experiment Videos

Last Updated: Feb 17, 2026

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Published on: May 23, 2025

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Published on: March 16, 2022

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Author Spotlight: A Novel Protocol for Intracameral Injections to Enhance Precision in Rodent Ophthalmology

Published on: May 31, 2024

Area of Science:

Ophthalmology
Regenerative Medicine
Biotechnology

Background:

Inherited eye diseases necessitate localized therapeutic molecule delivery via intraocular injection.
The efficacy of intraocular administration significantly impacts study outcomes.
The eye's vitreous compartment enables sustained release of biological therapeutics through intravitreal delivery.

Purpose of the Study:

To provide a detailed protocol for transscleral pars plana intravitreal transfer in small eyes.
To utilize a genetically modified stem cell system for therapeutic delivery.
To assess and refine intravitreal delivery techniques for enhanced treatment outcomes.

Main Methods:

Development of a transscleral pars plana intravitreal transfer protocol for small eyes.
Employment of a genetically modified stem cell system for implantation.
Utilizing visually distinct cells/particles for site and distribution assessment.

Main Results:

Successful implantation of genetically modified stem cells in small eyes.
Demonstrated ability to track cell distribution and implantation sites.
Established a method for temporal follow-up studies to monitor therapeutic delivery.

Conclusions:

The described protocol enables precise intravitreal delivery of therapeutics in small eyes.
Genetically modified stem cells serve as effective carriers for assessing and optimizing delivery methods.
This technique provides valuable data for refining intravitreal delivery strategies for inherited eye diseases.