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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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[Correlation between RNA Expression Level and Early PMI in Human Brain Tissue].

Y H Lü1, K J Ma2, Z H Li1

  • 1Shanghai University of Medicine & Health Science, Shanghai 201318, China.

Fa Yi Xue Za Zhi
|December 1, 2017
PubMed
Summary
This summary is machine-generated.

Stable RNA markers 5S rRNA, miRNA-9, and miRNA-125b in human brain tissue can help estimate early postmortem interval (PMI). Beta-actin expression also correlates with PMI, serving as an additional index for forensic analysis.

Keywords:
RNAbrainforensic pathologypostmortem intervalreal-time fluorescent quantitative PCR

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Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genomics

Background:

  • Accurate postmortem interval (PMI) estimation is crucial in forensic investigations.
  • RNA degradation patterns in brain tissue offer potential biomarkers for PMI determination.
  • Identifying stable RNA reference markers is essential for reliable molecular analysis.

Purpose of the Study:

  • To investigate the correlation between RNA marker expression levels and early postmortem interval (PMI) in human brain tissue.
  • To identify suitable internal reference RNA markers for accurate PMI estimation.
  • To evaluate the utility of specific RNA markers in forensic science.

Main Methods:

  • Total RNA was extracted from human brain tissue of individuals with known PMI (4.3–22.5 hours).
  • Expression levels of eight RNA markers (including 5S rRNA, miRNA-9, miRNA-125b, β-actin, GAPDH) were quantified using real-time PCR.
  • GeNorm software was used to screen for stable internal reference markers, and their relationship with age, gender, and cause of death was analyzed.

Main Results:

  • 5S rRNA, miRNA-9, and miRNA-125b exhibited stable expression, independent of age, gender, or cause of death.
  • The error rate for PMI estimation using β-actin was 24.6%, while GAPDH showed a higher error rate of 41.0%.
  • The expression levels of these stable RNA markers were suitable for normalizing gene expression in early PMI studies.

Conclusions:

  • 5S rRNA, miRNA-9, and miRNA-125b are validated as reliable internal reference markers for human brain tissue in early PMI estimations.
  • The expression level of β-actin shows a significant correlation with PMI and can serve as an additional index for early PMI determination.
  • These findings enhance the molecular toolkit for forensic scientists in estimating time since death.