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[Review of Second Generation Sequencing and Its Application in Forensic Genetics]
S H Zhang1, Y N Bian1, Q Zhao1
1Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China.
Fa Yi Xue Za Zhi
|December 1, 2017
Summary
Second-generation sequencing (SGS) offers breakthroughs in forensic genetics by enabling advanced genetic marker detection. Further development of kits, software, and ethical considerations are crucial for its widespread adoption in case detection.
Area of Science:
- Forensic Genetics
- Molecular Biology
- Genomics
Background:
- Second-generation sequencing (SGS) has rapidly advanced, increasing data throughput and read length while reducing costs.
- This technological progress has significantly impacted biological research and introduced new possibilities in forensic genetics.
Purpose of the Study:
- To review the historical application of sequencing in forensic genetics.
- To discuss the current status and future potential of SGS in forensic genetic marker detection.
Main Methods:
- Review of existing literature on sequencing technologies in forensic genetics.
- Analysis of applications using established SGS platforms (Roche, Illumina, Life Technologies).
- Examination of marker types amenable to SGS (DNA SNPs, STRs; RNA mRNA, microRNA; mtDNA).
Main Results:
- SGS platforms enable sequencing of various genetic markers, including DNA, RNA, and mitochondrial DNA.
- The potential for SGS in forensic genetics is significant, covering diverse marker types.
Conclusions:
- Widespread adoption of SGS in forensic genetics hinges on the development of application kits and analysis software.
- Integration with existing databases and addressing ethical concerns related to big data are critical for SGS to complement or replace current PCR-CE technologies.
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