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Shotgun Sphingolipid Analysis of Human Aqueous Humor
Anna Trzeciecka1, Ulises Arbelo2, Arturo Barron2
1Bascom Palmer Eye Institute, McKnight Vision Research Center, University of Miami, Miami, FL, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 1, 2017
Abstract:
This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.
