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Lysosome instability in aged rat brain.

Y Nakamura1, M Takeda, H Suzuki

  • 1Department of Neuropsychiatry, Osaka University Medical School, Japan.

Neuroscience Letters
|February 13, 1989
PubMed
Summary
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Lysosomal enzyme activity changes with age. Aged rat brains showed increased cathepsin D and lysosomal enzyme leakage, indicating lysosomal instability.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Gerontology

Background:

  • Lysosomes are crucial for cellular waste disposal.
  • Age-related changes in brain lysosomes are not fully understood.
  • Lysosomal enzyme activity may be altered during brain aging.

Purpose of the Study:

  • To investigate age-dependent changes in lysosomal enzyme activities in rat cerebral tissue.
  • To examine the subcellular distribution of specific lysosomal enzymes in aged brains.
  • To assess lysosomal membrane stability in aged rat brain tissue.

Main Methods:

  • Assayed lysosomal enzyme activities (cathepsin D, beta-glucuronidase, acid phosphatase) in aged rat cerebral tissue.
  • Performed subcellular fractionation to determine enzyme distribution.

Related Experiment Videos

  • Conducted in vitro lysosome incubation to measure enzyme leakage.

    • Main Results:

    • Cathepsin D activity significantly increased in aged rat brains; beta-glucuronidase and acid phosphatase remained unchanged.
    • Increased cathepsin D and beta-glucuronidase activity observed in the cytosolic fraction of aged brains.
    • Lysosomes from aged rat brains exhibited increased enzyme leakage in vitro, similar to L-Leucine methyl ester effects.

    Conclusions:

    • Aged rat brains exhibit altered lysosomal enzyme profiles, with increased cathepsin D.
    • Lysosomal instability and increased enzyme leakage are characteristic of aging brain tissue.
    • These findings suggest impaired lysosomal function contributes to brain aging.