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Updated: Feb 17, 2026
Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection
Published on: May 13, 2019
A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold
Fei Qu1, Wei Huang1, Jinmao You2
1The Key Laboratory of Life-Organic Analysis, Qufu Normal University, Qufu 273165, Shandong, China; The Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University, Qufu 273165, Shandong, China.
Abstract:
In this work, we report a fluorescence strategy for detecting dopamine (DA) and sensing tyrosinase (TYR) activity on the basis of the dual-emission carbon dots (DECDs), which contain two emitters: the blue emitters (BE, maximum emission at 385nm) and yellow emitters (YE, maximum emission at 530nm). Gold nanoparticles (AuNPs) can effectively quench the two emissions of DECDs. The addition of DA aggregates AuNPs effectively, leading to the fluorescence recovery of dual emitters gradually. This strategy exhibits a high selectivity toward DA and shows good linear ranges, such as 0.5-3μM for BE and 0.1-3μM for YE. Additionally, the proposed method is successfully applied to the determination of DA in real samples with satisfactory recoveries. Subsequently, this DECDs-AuNPs platform is further taken advantage to assess TYR activity by the aid of TYR's capability for oxidation of DA into dopaquinone, which will not induce the agglomeration of AuNPs, so the fluorescence quenching of DECDs is associated with TYR activity. Finally, the mechanism of the reaction is discussed in detail, and the results suggest that both amine and phenolic hydroxyl groups of DA bring the aggregation of AuNPs.
