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Updated: Feb 17, 2026

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish
Published on: April 30, 2013
GABAA receptors activate fish feeding behaviour via two distinct functional pathways
Sergey Snigirov1, Sergiy Sylantyev2,3
1Biological Department, Odessa National Mechnikov University, 2 Shampanskiy Lane, Odessa 65058, Ukraine.
Abstract:
Benzodiazepines, acting through ionotropic receptors of γ-aminobutyric acid (GABAA receptors, GABAR), have been shown to modify feeding behaviour and increase appetite in humans and non-human subjects. However, the cellular and molecular mechanisms that underlie connected short-term behavioural fluctuations are still unclear. In the present study, we used Carassius gibelio (Prussian carp) as a model organism to research the impact of scantily explored benzodiazepine phenazepam (PNZ) on feeding behaviour and the related molecular mechanisms of PNZ action at single-cell and single-receptor levels. We found that the feeding activity of C. gibelio is under control of GABARs via two distinct mechanisms: orthosteric (triggered by GABA binding site) and allosteric (triggered by benzodiazepine binding site). PNZ displayed clear stimulatory effects on both mechanisms in a GABA-dependent manner. In addition, orthosteric and allosteric effects were found to be partially competitive, which leads to complex behavioural repercussions of conjoint effects of GABAR ligands.
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