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Updated: Feb 17, 2026
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
Prognostic significance of Ki67 and its correlation with mitotic index in dogs with diffuse large B-cell lymphoma
Oscar R Sierra Matiz1,2,3,4,5, Juliana Santilli1,2,3,4,5, Leticia A Anai1,2,3,4,5
1Clinical Veterinary Department, College of Agricultural and Veterinary Sciences, São Paulo State University 'Júlio de Mesquita Filho' (UNESP), Jaboticabal, São Paulo, Brazil (Sierra Matiz, Anai, Costa, Calazans).
Abstract:
Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma in dogs. We evaluated Ki67 immunoexpression and mitotic index (MI) in dogs diagnosed with DLBCL and treated with a 19-wk CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) protocol. Twenty-nine lymph node samples from dogs diagnosed with DLBCL were analyzed for Ki67 immunostaining, and positive cells present in 1 cm2 were counted in a grid reticle for comparison of survival times above and below the means. The Ki67 mean was 107, and the MI mean was 21. There was a significant ( p < 0.05) difference in median survival time between Ki67 immunostaining above and below the mean, with no difference in MI groups. Ki67 values >107 positive cells per 5 HPF counted in a grid reticle were associated with shorter survival times in dogs with DLBCL treated with a 19-wk CHOP-based protocol.
