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Related Experiment Videos

Arsenic-selenium interactions determined with cultured fish cells.

H Babich1, N Martin-Alguacil, E Borenfreund

  • 1Laboratory Animal Research Center, Rockefeller University, New York, NY 10021.

Toxicology Letters
|February 1, 1989
PubMed
Summary

Bluegill sunfish (Lepomis macrochirus) cell lines showed that arsenite and arsenate were more toxic than selenite and selenate. Selenium compounds reduced the toxicity of arsenic compounds in these aquatic bioindicators.

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Area of Science:

  • Environmental Toxicology
  • Aquatic Toxicology
  • Cell Biology

Background:

  • Bluegill sunfish (Lepomis macrochirus) are important aquatic bioindicators.
  • Cell lines derived from fish tissues allow for controlled in vitro toxicity testing.
  • Arsenic and selenium are common environmental contaminants with known toxic effects.

Purpose of the Study:

  • To evaluate the comparative cytotoxicity of arsenic and selenium compounds using bluegill sunfish cell lines.
  • To investigate potential interactions between arsenic and selenium in aquatic organisms.
  • To establish NR50 (neutral red uptake inhibition concentration) values for these contaminants.

Main Methods:

  • Established two cell lines from bluegill sunfish: BG/G (gill) and BG/F (fin).

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  • Utilized the neutral red cytotoxicity assay as the primary endpoint.
  • Exposed both cell lines to varying concentrations of arsenite, arsenate, selenite, and selenate.

    • Main Results:

    • Cytotoxicity ranking was consistent across both cell lines: arsenite > arsenate > selenite > selenate.
    • NR50 concentrations were comparable between the BG/G and BG/F cell lines.
    • Non-toxic levels of selenate and selenite demonstrated an antagonistic effect, reducing the acute cytotoxicity of arsenate and arsenite.

    Conclusions:

    • Bluegill sunfish cell lines are reliable bioindicators for assessing arsenic and selenium toxicity.
    • Selenium compounds can mitigate the toxic effects of arsenic compounds in aquatic species.
    • Understanding these interactions is crucial for environmental risk assessment of mixed contaminant exposures.