Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy.

International journal of molecular sciences·2021

Same author

Isolation of pathogenic bacteria from sputum samples using a 3D-printed cartridge system.

Analytical methods : advancing methods and applications·2021

Same author

A Study in Red: The Overlooked Role of Azo-Moieties in Polymeric Carbon Nitride Photocatalysts with Strongly Extended Optical Absorption.

Chemistry (Weinheim an der Bergstrasse, Germany)·2021

Same author

Infrared Refraction Spectroscopy.

Applied spectroscopy·2021

Same author

A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga.

Proceedings of the National Academy of Sciences of the United States of America·2021

Same author

Looking for a perfect match: multimodal combinations of Raman spectroscopy for biomedical applications.

Journal of biomedical optics·2021

Search research articles

Related Experiment Video

Updated: Feb 17, 2026

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

Published on: September 13, 2019

Tracking active groundwater microbes with D₂ O labelling to understand their ecosystem function.

Martin Taubert¹, Stephan Stöckel², Patricia Geesink¹

¹Aquatic Geomicrobiology, Institute of Biodiversity, Friedrich Schiller University Jena, Dornburger Str. 159, 07743 Jena, Germany.

Environmental Microbiology

|December 2, 2017

Summary

This summary is machine-generated.

Heavy water (D2O) metabolic labeling reveals microbial activity in groundwater. Raman microspectroscopy and metaproteomics identified specific microbes and pathways responding to different organic compounds.

More Related Videos

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Published on: March 24, 2023

Bromodeoxyuridine BrdU Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Bromodeoxyuridine BrdU Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Published on: September 10, 2011

Related Experiment Videos

Last Updated: Feb 17, 2026

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems

Published on: September 13, 2019

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Dissolved Solute Sampling Across an Oxic-Anoxic Soil-Water Interface Using Microdialysis Profilers

Published on: March 24, 2023

Bromodeoxyuridine BrdU Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Bromodeoxyuridine BrdU Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Published on: September 10, 2011

Area of Science:

Microbiology
Environmental Science
Biogeochemistry

Background:

Microbial communities drive ecosystem functions.
Metabolic activity is crucial for understanding microbial contributions.
Heavy water (D2O) labeling can track active microorganisms.

Purpose of the Study:

To demonstrate D2O labeling for monitoring microbial metabolic activity.
To functionally characterize active microbial populations in complex communities.
To elucidate microbial responses to different organic substrates.

Main Methods:

Single-cell Raman microspectroscopy for cell labeling verification.
D2O labeling experiments in groundwater microcosms.
Metaproteomics for identifying active microbial players and pathways.

Main Results:

D2O labeling confirmed metabolic activity in groundwater microbes.
Heterotrophs assimilating veratric acid showed higher D2O incorporation than those using methylamine.
Sphingomonadaceae and Microbacteriaceae were key in veratric acid degradation.
Methylamine stimulated diverse proteobacterial genera.

Conclusions:

Combined D2O labeling, Raman microspectroscopy, and metaproteomics is effective for studying microbial metabolism.
Substrate type influences microbial activity and community composition.
This approach provides insights into microbial responses in complex environments.