Ontogeny of Surface Texture of Limb Bones in Modern Aquatic Birds and Applicability of Textural Ageing

Despite its importance in various disciplines, a general method to assess ontogenetic ages of skeletal and fossil specimens has been lacking for birds. Although the textural ageing method was formulated to assess relative ontogenetic ages of specimens from inspection of bone surface textures, the exact correspondence of surface textures to ontogenetic stages has not yet been clear. In this study, bone surface textures of six major limb bones (humerus, ulna, carpometacarpus, femur, tibiotarsus, and tarsometatarsus) were described in postnatal ontogenies of four species of modern birds (Calonectris leucomelas, Phalacrocorax capillatus, Larus crassirostris, and Cerorhinca monocerata) from 14 to 28 individuals of known ontogenetic stages for each species. Consistently with the previous postulation, it was found that bones of chicks were characterized by rough surface textures with numerous grooves/depressions that host minute foramina. Bones of fledglings/juveniles, which are generally as large as those of adults but more slender, were characterized by the occasional presence of depressions and foramina. Histological observations confirmed that these rough surface textures were underlain by fibrolamellar bone tissue which is associated with active periosteal ossification. These results indicate that the smooth surface texture in adults is formed after the cessation of circumferential bone growth, which probably takes place between fledging and the attainment of sexual maturity. The available evidence suggests that the textural ageing is probably applicable to the entire Neognathae, a clade containing most crown-group birds. Anat Rec, 301:1026-1045, 2018. © 2017 Wiley Periodicals, Inc.