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Essential Role of IFT140 in Promoting Dentinogenesis
11 Department of Implantology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.
Journal of Dental Research
|December 2, 2017
Summary
Intraflagellar transport 140 (IFT140) is crucial for tooth development and repair. Loss of IFT140 impairs dentin formation and regeneration by affecting primary cilia in odontoblasts.
Area of Science:
- Cell Biology
- Developmental Biology
- Genetics
Background:
- Primary cilia are vital sensory organelles regulating tissue development and function.
- Intraflagellar transport 140 (IFT140) is a key component of retrograde intraflagellar transport within cilia.
- IFT140 mutations are linked to ciliopathies, but its role in tooth development is unexplored.
Purpose of the Study:
- To investigate the role of IFT140 in dentinogenesis and tooth development.
- To elucidate the function of IFT140 in odontoblasts and their differentiation.
- To assess the impact of IFT140 on reparative dentin formation.
Main Methods:
- Generated Ift140flox/flox/Osx-Cre conditional knockout mice.
- Analyzed dentin thickness and formation rate in knockout mice.
- Performed in vitro studies on odontoblasts lacking IFT140.
- Utilized a tooth-drilling model to assess reparative dentin formation.
Main Results:
- IFT140 is highly expressed in odontoblasts during tooth development.
- Deletion of IFT140 resulted in thinner dentin and slower dentin formation.
- IFT140-deficient odontoblasts showed impaired differentiation, abnormal cilia, and reduced Sonic hedgehog signaling.
- Loss of IFT140 and primary cilia in odontoblasts significantly impaired reparative dentin formation.
Conclusions:
- IFT140 is essential for normal dentin formation and development.
- Primary cilia, regulated by IFT140, are critical for odontoblast function and dentinogenesis.
- IFT140 plays a vital role in the capacity for tooth repair through enhanced dentin formation.
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