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Updated: Jul 27, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Hand-held synchronous scan spectrometer for in situ and immediate detection of live/dead bacteria ratio
Runze Li1, Umang Goswami1, Matthew Walck1
1Department of Electrical and Computer Engineering, Texas A&M University, College Station, Texas 77843, USA.
Abstract:
The design, construction, and operation of a hand-held synchronously scanned, excitation-emission, double monochromator spectrometer is described. Data show that it is possible to record and display within minutes the fluorescence spectra and ratio of live/dead bacteria in situ. Excitation emission matrix contour plots display clearly bacteria fluorescence spectra before and after UV inactivation, respectively. The separation of the fluorescence band maxima of molecular components, such as tryptophan, tyrosine, and DNA, may be distinguished in the diffused fluorescence spectra of bacteria and mixtures.
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