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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
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Paving TRAIL's Path with Ubiquitin.

Elodie Lafont1, Torsten Hartwig1, Henning Walczak2

  • 1Centre for Cell Death, Cancer, and Inflammation, UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6DD, UK; These authors contributed equally to this work.

Trends in Biochemical Sciences
|December 3, 2017
PubMed
Summary
This summary is machine-generated.

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) signaling is versatile, controlling cell death, migration, and proliferation. Ubiquitination regulates these diverse TRAIL signaling outcomes, impacting immunity and cancer.

Keywords:
LUBACTRAILcytokinedeathinflammationubiquitin

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Signaling by tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is multifaceted.
  • TRAIL induces not only cell death (apoptosis, necroptosis) but also cell migration, proliferation, and cytokine production.
  • Understanding TRAIL's diverse roles in tissue homeostasis, immunity, and cancer is crucial.

Purpose of the Study:

  • To review recent advancements in understanding TRAIL signaling.
  • To elucidate the role of ubiquitination and deubiquitination in modulating TRAIL signaling outcomes.
  • To identify key molecular switches governing TRAIL-mediated cell death versus gene activation.

Main Methods:

  • Literature review of recent progress in TRAIL signaling research.
  • Focus on the role of ubiquitination in TRAIL signaling complexes.
  • Analysis of molecular mechanisms controlling TRAIL pathway decisions.

Main Results:

  • Ubiquitination and deubiquitination are critical regulators of TRAIL signaling.
  • These post-translational modifications orchestrate diverse cellular responses to TRAIL.
  • Specific ubiquitination patterns dictate whether TRAIL signaling leads to cell death or gene activation.

Conclusions:

  • TRAIL signaling outcomes are intricately regulated by ubiquitination.
  • Further research into these mechanisms can illuminate TRAIL's roles in disease.
  • Identifying molecular switches is key to understanding and potentially manipulating TRAIL functions.