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Spatial (cap & stipe) metabolomic variations affect functional components between brown and white beech mushrooms.

Yu Jin Park1, Eun Sung Jung1, Digar Singh1

  • 1Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Republic of Korea.

Food Research International (Ottawa, Ont.)
|December 3, 2017
PubMed
Summary

Beech mushrooms show distinct metabolite profiles in caps versus stipes and between brown and white strains. These differences, including cytotoxic hypsiziprenols, aid mushroom selection and improvement.

Keywords:
Beech mushroomsCytotoxic activityGC-TOF-MSHypsiziprenolMetabolite profilingUHPLC-MS/MS

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Area of Science:

  • * Food Science
  • * Mycology
  • * Metabolomics

Background:

  • * Beech mushrooms are valued in oriental societies for health and nutrition.
  • * Understanding their chemical composition is key to unlocking their full potential.

Purpose of the Study:

  • * To investigate spatial metabolomic variations within beech mushroom parts (cap and stipe).
  • * To compare metabolite profiles between brown and white beech mushroom strains.
  • * To correlate specific metabolites with cytotoxic activities.

Main Methods:

  • * Mass spectrometry (MS) based spatial metabolomics.
  • * Principal Component Analysis (PCA) for metabolite pattern analysis.
  • * Cytotoxicity assays against ACHN cells.

Main Results:

  • * Distinct primary and secondary metabolite patterns were identified between caps and stipes, and between strains.
  • * Caps were rich in amino acids, fatty acids, and N-acetylglucosamine; stipes contained abundant β-glucans, malic acid, and fructose.
  • * Brown strains had higher hypsiziprenols in caps, while white strains showed more azelaic acid.

Conclusions:

  • * Spatial and inter-strain metabolomic differences in beech mushrooms are significant.
  • * Hypsiziprenols exhibit cytotoxic activity against ACHN cells.
  • * These findings can guide selection and improvement of beech mushroom strains for specific applications.