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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...

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Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Related Experiment Video

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

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Clinical proteomics: Insights from IGF-I.

Jakob Albrethsen¹, Hanne Frederiksen¹, Trine Holm Johannsen¹

¹Dept. of Growth and Reproduction, Rigshospitalet, University of Copenhagen, Denmark; International Center for Research and Research Training in Endocrine, Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, Denmark.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|December 3, 2017

Summary

Targeted proteomics offers a cost-effective way to measure diagnostic proteins like insulin-like growth factor 1 (IGF-I). Researchers are evaluating if this mass spectrometry method meets clinical testing standards for routine use.

Keywords:

IGF-I Mass spectrometry Plasma Proteomics

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Area of Science:

Clinical proteomics
Analytical chemistry
Mass spectrometry

Background:

Clinical proteomics seeks cost-effective multiplexing of diagnostic proteins and isoforms.
Targeted proteomics is compatible with existing mass spectrometry (MS) platforms in clinical labs.
Previous work has advanced targeted proteomics for circulating insulin-like growth factor 1 (IGF-I).

Purpose of the Study:

To assess the suitability of targeted proteomics for routine clinical measurement of IGF-I.
To determine if IGF-I targeted proteomics can meet clinical testing performance expectations.

Main Methods:

Utilizing targeted proteomics, a mass spectrometry-based analytical strategy.
Leveraging ultra-high-performance liquid chromatography (uHPLC) coupled to triple-quadrupole MS.
Focusing on tryptic peptides, transitions, internal standards, and calibrants for IGF-I quantitation.

Main Results:

Valuable insights have been gained regarding tryptic peptides, transitions, internal standards, and calibrants for IGF-I.
The study examines the practical application and performance of IGF-I targeted proteomics.

Conclusions:

Targeted proteomics shows promise for clinical diagnostics.
Further evaluation is needed to confirm IGF-I targeted proteomics meets routine clinical performance standards.